Project information
Phenotypic plasticity of monogenean parasites: a combined molecular and morphological approach
- Project Identification
- GA524/07/1610
- Project Period
- 1/2007 - 12/2010
- Investor / Pogramme / Project type
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Czech Science Foundation
- Standard Projects
- MU Faculty or unit
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Faculty of Science
- RNDr. Božena Koubková, Ph.D.
- prof. RNDr. Milan Gelnar, CSc.
- Mgr. Iveta Hodová, Ph.D.
- Mgr. Eva Košková
- Mgr. Iveta Matějusová, Ph.D.
- Mgr. Monika Mendlová, Ph.D.
- Mgr. Martina Pečínková, Ph.D.
- Mgr. Radim Sonnek
- Mgr. Amálie Zehnalová
- Keywords
- Monogenea, Diplozoidae, phenotypic plasticity, morphometry, chromosome, cytogenetics, molecular genetics, IGS, ITS
Species discrimination of diplozoids, based on morphology of the attachment apparatus, is difficult and demands a great deal of experience as these parts are highly variable. Species markers based on the ribosomal rRNA gene array will be designed for diplozoids belonging to various species complexes and the validity of recent morphological and characters for species identification will be evaluated. Also new methods of molecular karyology including data on macro and micromorphology of chromosomes will be used for evaluation of inter and intraspecific structure of selected taxa. Parasite specimens of Paradiplozoon homoion from different European, Mediterranean and Far East populations will be collected and differences in the intergenic spacer rRNA genes (IGS) will be analysed. Also populations of P. homoion from different host fish will screened for differences in the IGS to reveal possible host related selection pressures leading to speciation.
Publications
Total number of publications: 17
2011
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Comparative karyological analysis of four diplozoid species (Monogenea, Diplozoidae), gill parasites of cyprinid fishes
Parasitology Research, year: 2011, volume: 108, edition: 4, DOI
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Eudiplozoon nipponicum in focus: monogenean exhibiting a highly specialized adaptation for ectoparasitic lifestyle
Parasitology Research, year: 2011, volume: 108, edition: 2, DOI
2010
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Ethanol-fixed material used for both classical and molecular identification purposes: Eudiplozoon nipponicum (Monogenea: Diplozoidae) as a case parasite species
Parasitology Research, year: 2010, volume: 107, edition: 4
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Eudiplozoon nipponicum in focus: monogenean parasite exhibiting a highly specialized adaptation for ectoparasitic life.
Year: 2010, type: Conference abstract
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Kombinace morfologických přístupů k vyhodnocení ontogenetických a fylogenetických adaptací diplozoonů (Monogenea) k ektoparazitickému způsobu života
Year: 2010, type: Conference abstract
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Modified sample preparation from ethanol fixed material for identification of Eudiplozoon nipponicum as a case parasite species for the combined morphological and molecular characterization
Year: 2010, type: Conference abstract
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Morphological study of adult parasite Eudiplozoon nipponicum by different microscopic techniques
Year: 2010, type: Conference abstract
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The surface topography of Eudiplozoon nipponicum (Monogenea) developmental stages parasitizing carp (Cyprinus carpio L.)
Central European Journal of Biology, year: 2010, volume: 5, edition: 5
2009
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A new species of diplozoid parasite described from Iranian fish Rutilus frisii kutum.
Year: 2009, type: Conference abstract
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Analysis of significant surface structures of selected parasite taxa using the scanning eletron microscopy
Year: 2009, type: Conference abstract